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Early days of tRNA research: Discovery, function, purification and sequence analysis

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References

  • Anderson K W and Smith J D 1972 Still more mutant tyrosine transfer ribonucleic acids;J. Mol. Biol. 69 349–356

    Article  CAS  PubMed  Google Scholar 

  • Berg P 1956 Acyl adenylates; the interaction of adenosine triphosphate and L-methionine;J. Biol. Chem. 222 1025–1034

    CAS  PubMed  Google Scholar 

  • Berg P 1958 Studies on the enzymatic utilization of amino acyladenylates; the formation of adenosine triphosphate;J. Biol. Chem. 233 601–607

    CAS  PubMed  Google Scholar 

  • Berg P 2003 Moments of discovery: My favorite experiments;J. Biol. Chem. 278 40417–40424

    Article  CAS  PubMed  Google Scholar 

  • Berg P, Bergmann F H, Ofengand E J and Dieckmann M 1961 The enzymic synthesis of amino acyl derivatives of ribonucleic acid. I. The mechanism of leucyl-, valyl-, isoleucyl-, and methionyl ribonucleic acid formation;J. Biol. Chem. 236 1726–1734

    CAS  Google Scholar 

  • Berg P and Ofengand E J 1958 An enzymatic mechanism for linking amino acids to RNA;Proc. Natl. Acad. Sci. USA 44 78–86

    Article  CAS  PubMed  Google Scholar 

  • Canellakis E S 1957 On the mechanism of incorporation of adenylic acid form adenosine triphosphate into ribonucleic acid by soluble mammalian enzyme systems;Biochim. Biophys. Acta 25 217–218

    Article  CAS  PubMed  Google Scholar 

  • Chang S H, Brum C K, Siberklang M, RajBhandary U L, Hecker L I and Barnett W E 1976 The first nucleotide sequence of an organelle transfer RNA: Chloroplastic tRNAPhe;Cell 9 717–723

    Article  CAS  PubMed  Google Scholar 

  • Craig L C 1952 Countercurrent distribution;Methods Med. Res. 5 3–24

    CAS  PubMed  Google Scholar 

  • Crick F H 1966 Codon-anticodon pairing: The wobble hypothesis;J. Mol. Biol. 19 548–555

    Article  CAS  PubMed  Google Scholar 

  • Davie E W, Koningsberger V V and Lipmann F 1956 The isolation of a tryptophan-activating enzyme from pancreas;Arch. Biochem. Biophys. 65 21–38

    Article  CAS  PubMed  Google Scholar 

  • DeMoss J A, Genuth S M and Novelli G D 1956 The enzymatic activation of amino acids via their acyl-adenylate derivatives;Proc. Natl. Acad. Sci. USA 42 325–332

    Article  CAS  PubMed  Google Scholar 

  • DeMoss J A and Novelli G D 1956 An amino acid dependent exchange between32P labeled inorganic pyrophosphate and ATP in microbial extracts;Biochim. Biophys. Acta 22 49–61

    Article  CAS  PubMed  Google Scholar 

  • Dube S K, Marcker K A, Clark B F and Cory S 1968 Nucleotide sequence of N-formyl-methionyl-transfer RNA;Nature (London) 218 232–233

    Article  CAS  Google Scholar 

  • Dubois D Y, Blaise M, Becker H D, Campanacci V, Keith G, Giegé R, Cambillau C, Lapointe J and Kern D 2004 An aminoacyl—tRNA synthetase-like protein encoded by theEscherichia coli yadB gene glutamylates specifically tRNAAsp;Proc. Natl. Acad. Sci. USA 101 7530–7535

    Article  CAS  PubMed  Google Scholar 

  • Gillam I, Millward S, Blew D, von Tigerstrom M, Wimmer E and Tener G M 1967 The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose;Biochemistry 6 3043–3056

    Article  CAS  PubMed  Google Scholar 

  • Goodman H M, Abelson J, Landy A, Brenner S and Smith J D 1968 Amber suppression: A nucleotide change in the anticodon of a tyrosine transfer RNA;Nature (London) 217 1019–1024

    Article  CAS  Google Scholar 

  • Gupta R C and Randerath K 1979 Rapid print-readout technique for sequencing of RNA’s containing modified nucleotides;Nucleic Acids Res. 6 3443–3458

    Article  CAS  PubMed  Google Scholar 

  • Hecht L I, Zamecnik P C, Stephenson M L and Scott J. F 1958a Nucleoside tri-phosphates as precursors of ribonucleic acid end groups in a mammalian system;J. Biol. Chem. 233 954–963

    CAS  PubMed  Google Scholar 

  • Hecht L I, Stephenson M L and Zamecnik P C 1958b Dependence of amino acid binding to soluble ribonucleic acid on cytidine triphosphate;Biochim. Biophys. Acta 29 460–461

    Article  CAS  PubMed  Google Scholar 

  • Heckman J E, Hecker L I, Schwartzbach S D, Barnett W E, Baumstark B and RajBhandary U L 1978 Structure and function of initiator methionine tRNA from the mitochondria ofNeurospora crassa;Cell 13 83–95

    Article  CAS  PubMed  Google Scholar 

  • Heidelberger C, Harbers E, Leibman K C, Takagi Y and Potter V R 1956 Specific incorporation of adenosine-5’phosphate-32P into ribonucleic acid in rat liver homogenates;Biochim. Biophys. Acta 20 445–446

    Article  CAS  PubMed  Google Scholar 

  • Hoagland M 1996 Biochemistry or molecular biology? The discovery of ‘soluble RNA’;Trends Biochem. Sci. 21 77–80

    Article  CAS  PubMed  Google Scholar 

  • Hoagland M B, Keller E B and Zamecnik PC 1956 Enzymatic carboxyl activation of amino acids;J. Biol. Chem. 218 345–358

    CAS  PubMed  Google Scholar 

  • Hoagland M B, Stephenson M L, Scott J F, Hecht LI and Zamecnik P C 1958 A soluble ribonucleic acid intermediate in protein synthesis;J. Biol. Chem. 231 241–257

    CAS  PubMed  Google Scholar 

  • Hoagland M B, Zamecnik P C and Stephenson M L 1957 Intermediate reactions in protein biosynthesis;Biochim. Biophys. Acta 24 215–216

    Article  CAS  PubMed  Google Scholar 

  • Holley R 1957 An alanine-dependent, ribonuclease-inhibited conversion of AMP to ATP and its possible relationship to protein synthesis;J. Am. Chem. Soc. 79 658–661

    Article  CAS  Google Scholar 

  • Holley R W, Apgar J, Everett G A, Madison J T, Marquisee M, Merrill S H, Penswick J. R and Zamir A 1965 Structure of a ribonucleic acid;Science 147 1462–1465

    Article  CAS  PubMed  Google Scholar 

  • Holmes W M Hurd R E, Reid B R, Rimerman R A and Hatfield G W 1975 Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients;Proc. Natl. Acad. Sci. USA 72 1068–1071

    Article  CAS  PubMed  Google Scholar 

  • Kim S H, Suddath F L, Quigley G J, McPherson A, Sussman J L, Wang A H, Seeman N C and Rich A 1974 Three-dimensional tertiary structure of yeast phenylalanine transfer RNA.Science 185 435–440

    Article  CAS  PubMed  Google Scholar 

  • Kuchino Y, Kato M, Sugisaki H and Nishimura S 1979 Nucleotide sequence of starfish initiator tRNA;Nucleic Acids Res. 6 3459–3469

    Article  CAS  PubMed  Google Scholar 

  • Lipmann F, Hülsmann W C, Hartmann G, Boman H G and Acs G 1959 Amino acid activation and protein synthesis;J. Cell Comp. Physiol. 54 75–88

    Article  CAS  PubMed  Google Scholar 

  • Litt M and Ingram V M 1964 Chemical studies on amino acid acceptor ribonucleic acids. II. Attempts at partial digestion of yeast amino acid acceptor ribonucleic acid with pancreatic ribonuclease;Biochemistry 3 560–564

    Article  CAS  PubMed  Google Scholar 

  • Madison J T, Everett G A and Kung H 1966 Nucleotide sequence of a yeast tyrosine transfer RNA;Science 153 531–534

    Article  CAS  PubMed  Google Scholar 

  • Maxam A M and Gilbert W 1977 A new method for sequencing DNA;Proc. Natl. Acad. Sci. USA 74 560–564

    Article  CAS  PubMed  Google Scholar 

  • Nishimura S, Harada F, Narushima U and Seno T 1967 Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA fromEscherichia coli;Biochim. Biophys. Acta 142 133–148

    CAS  PubMed  Google Scholar 

  • Nishimura S and Novelli G D 1963 Resistance of S-RNA to ribonucleases in the presence of magnesium ion;Biochem. Biophys. Res. Commun. 11 161–165

    Article  CAS  PubMed  Google Scholar 

  • Ogata K and Nohara H 1957 The possible role of the ribonucleic acid (RNA) of the pH 5 enzyme in amino acid activation;Biochim. Biophys. Acta 25 659–660

    Article  CAS  PubMed  Google Scholar 

  • Pearson R L, Weiss J F and Kelmers A D 1971 Improved separation of transfer RNA’s on polychlorotrifuoroethylene-supported reversed-phase chromatography columns;Biochim. Biophys. Acta 228 770–774

    CAS  PubMed  Google Scholar 

  • Peattie D A 1979 Direct chemical method for sequencing RNA;Proc. Natl. Acad. Sci. USA 76 1760–1764

    Article  CAS  PubMed  Google Scholar 

  • RajBhandary U L 1980 Recent developments in methods for RNA sequencing usingin vitro 32P-labeling;Fed. Proc. 39 2815–2821

    CAS  PubMed  Google Scholar 

  • RajBhandary U L and Chang S H 1968 Studies on polynucleotides. LXXXII. Yeast phenylalanine transfer ribonucleic acid: Partial digestion with ribonuclease T-1 and derivation of the total primary structure;J. Biol. Chem. 243 598–608

    CAS  PubMed  Google Scholar 

  • RajBhandary U L, Chang S H, Stuart A, Faulkner R D, Hoskinson R M and Khorana H G 1967 Studies on polynucleotides. LXVIII. The primary structure of yeast phenylalanine transfer RNA;Proc. Natl. Acad. Sci. USA 57 751–758

    Article  CAS  PubMed  Google Scholar 

  • RajBhandary U L, Stuart A and Chang S H 1968 Studies on polynucleotides. LXXX. Yeast phenylalanine transfer ribonucleic acid: Products obtained by degradation with ribonuclease T1;J. Biol Chem. 243 584–591

    CAS  PubMed  Google Scholar 

  • RajBhandary U L, Stuart A, Faulkner R D, Chang S H and Khorana H G 1966 Nucleotide sequence studies on yeast phenylalanine sRNA;Cold Spring Harb. Symp. Quant. Biol. 31 425–434

    CAS  PubMed  Google Scholar 

  • Robertus J D, Ladner J E, Finch J T, Rhodes D, Brown R S, Clark B F and Klug A 1974 Structure of yeast phenylalanine tRNA at 3 Å resolution;Nature (London) 250 546–551

    Article  CAS  Google Scholar 

  • Sakurai M, Ohtsuki T, Suzuki T and Watanabe K 2005 Unusual usage of wobble modifications in mitochondrial tRNAs of the nematodeAscaris suum;FEBS Lett. 579 2767–2772

    Article  CAS  PubMed  Google Scholar 

  • Sanger F, Brownlee G G and Barrell B G 1965 A two-dimensional fractionation procedure for radioactive nucleotides;J. Mol. Biol. 13 373–398

    CAS  PubMed  Google Scholar 

  • Schweet R S, Bovard F C, Allen E and Glassman E 1958 The incorporation of amino acids into ribonucleic acids;Proc. Natl. Acad. Sci. USA 44 173–177

    Article  CAS  PubMed  Google Scholar 

  • Silberklang M, Gillum A M and RajBhandary U L 1979 Use ofin vitro 32P labeling in the sequence analysis of nonradioactive tRNAs;Methods Enzymol. 59 58–109

    Article  CAS  PubMed  Google Scholar 

  • Simoncsits A, Brownlee G G, Brown R S, Rubin J R and Guilley H 1977 New rapid gel sequencing method for RNA;Nature (London) 269 833–836

    Article  CAS  Google Scholar 

  • Söll D and RajBhandary U L 1967 Studies on polynucleotides. LXXVI. Specificity of transfer RNA for codon recognition as studied by amino acid incorporation;J. Mol. Biol. 29 113–124

    Article  PubMed  Google Scholar 

  • Stanley J and Vassilenko S 1978 A different approach to RNA sequencing;Nature (London) 274 87–89

    Article  CAS  Google Scholar 

  • Tomlinson R V and Tener G M 1963 The effect of urea, formamide, and glycols on the secondary binding forces in the ion-exchange chromatography of polynucleotides of DEAE-cellulose;Biochemistry 2 697–702

    Article  CAS  PubMed  Google Scholar 

  • Zachau H G, Acs G and Lipmann F 1958 Isolation of adenosine amino acid esters from a ribonuclease digest of soluble, liver ribonucleic acid;Proc. Natl. Acad. Sci. USA 44 885–889

    Article  CAS  PubMed  Google Scholar 

  • Zachau H G, Dütting D and Feldmann H 1966 The structures of two serine transfer ribonucleic acids;Hoppe Seylers Z. Physiol. Chem. 347 212–235

    CAS  PubMed  Google Scholar 

  • Zamecnik P C 2005 From protein synthesis to genetic insertion;Annu. Rev. Biochem. 74 1–28

    Article  CAS  PubMed  Google Scholar 

  • Zamecnik P C, Stephenson M L and Hecht L I 1958 Intermediate reactions in amino acid incorporation;Proc. Natl. Acad. Sci. USA 44 73–78

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Uttam L. RajBhandary.

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RajBhandary, U.L., Köhrer, C. Early days of tRNA research: Discovery, function, purification and sequence analysis. J Biosci 31, 439–451 (2006). https://doi.org/10.1007/BF02705183

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