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when toggle format what by license comment
May 5, 2014 at 22:26 comment added Mick Mnemonic My plan is to do something similar to what you suggest (deconvolve the two stains and threshold the resulting two images separately). However, deconvolution and thresholding (Otsu's method) will fail with the example image because of the artifact, which is why I'd like an answer that addresses the artifact removal problem.
May 5, 2014 at 15:00 history answered Felix Castor CC BY-SA 3.0