update: Copied material that was on the R wiki at http://rwiki.sciviews.org/doku.php?id=tips:graphics-base:2yaxes, link now broken: also available from the wayback machine
Two different y axes on the same plot
(some material originally by Daniel Rajdl 2006/03/31 15:26)
Please note that there are very few situations where it is appropriate to use two different scales on the same plot. It is very easy to mislead the viewer of the graphic. Check the following two examples and comments on this issue (example1, example2 from Junk Charts), as well as this article by Stephen Few (which concludes “I certainly cannot conclude, once and for all, that graphs with dual-scaled axes are never useful; only that I cannot think of a situation that warrants them in light of other, better solutions.”) Also see point #4 in this cartoon ...
If you are determined, the basic recipe is to create your first plot, set par(new=TRUE) to prevent R from clearing the graphics device, creating the second plot with axes=FALSE (and setting xlab and ylab to be blank – ann=FALSE should also work) and then using axis(side=4) to add a new axis on the right-hand side, and mtext(...,side=4) to add an axis label on the right-hand side. Here is an example using a little bit of made-up data:
set.seed(101) x <- 1:10 y <- rnorm(10) ## second data set on a very different scale z <- runif(10, min=1000, max=10000) par(mar = c(5, 4, 4, 4) + 0.3) # Leave space for z axis plot(x, y) # first plot par(new = TRUE) plot(x, z, type = "l", axes = FALSE, bty = "n", xlab = "", ylab = "") axis(side=4, at = pretty(range(z))) mtext("z", side=4, line=3)
twoord.plot() in the plotrix package automates this process, as does doubleYScale() in the latticeExtra package.
Another example (adapted from an R mailing list post by Robert W. Baer):
## set up some fake test data time <- seq(0,72,12) betagal.abs <- c(0.05,0.18,0.25,0.31,0.32,0.34,0.35) cell.density <- c(0,1000,2000,3000,4000,5000,6000) ## add extra space to right margin of plot within frame par(mar=c(5, 4, 4, 6) + 0.1) ## Plot first set of data and draw its axis plot(time, betagal.abs, pch=16, axes=FALSE, ylim=c(0,1), xlab="", ylab="", type="b",col="black", main="Mike's test data") axis(2, ylim=c(0,1),col="black",las=1) ## las=1 makes horizontal labels mtext("Beta Gal Absorbance",side=2,line=2.5) box() ## Allow a second plot on the same graph par(new=TRUE) ## Plot the second plot and put axis scale on right plot(time, cell.density, pch=15, xlab="", ylab="", ylim=c(0,7000), axes=FALSE, type="b", col="red") ## a little farther out (line=4) to make room for labels mtext("Cell Density",side=4,col="red",line=4) axis(4, ylim=c(0,7000), col="red",col.axis="red",las=1) ## Draw the time axis axis(1,pretty(range(time),10)) mtext("Time (Hours)",side=1,col="black",line=2.5) ## Add Legend legend("topleft",legend=c("Beta Gal","Cell Density"), text.col=c("black","red"),pch=c(16,15),col=c("black","red"))

Similar recipes can be used to superimpose plots of different types – bar plots, histograms, etc..
ggplot2: stackoverflow.com/questions/3099219/… (searching SO for[r] two y-axesor[r] twoord.plot) -- there are a few other related answers, although (to my surprise since it's an R FAQ) nothing identical