With the data separated by categories (Samples A and B), 2 layers were made, one for points and one for lines. I want to separate my data by category indicating colors for the points and also separate the lines but with different colors than those used for the points.
library(ggplot2) Sample <- c("a", "b") Time <- c(0,1,2) df <- expand.grid(Time=Time, Sample = Sample) df$Value <- c(1,2,3,2,4,6) ggplot(data = df, aes(x = Time, y = Value)) + geom_point(aes(color = Sample)) + geom_line(aes(color = Sample)) + scale_color_manual(values = c("red", "blue")) + #for poits scale_color_manual(values = c("orange", "purple")) #for lines 
